Ectodomain Shedding of Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE-1) Is Induced by Vascular Endothelial Growth Factor A (VEGF-A)*
Identifieur interne : 001155 ( Main/Exploration ); précédent : 001154; suivant : 001156Ectodomain Shedding of Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (LYVE-1) Is Induced by Vascular Endothelial Growth Factor A (VEGF-A)*
Auteurs : Hisayo Nishida-Fukuda ; Ryoichi Araki ; Masachika Shudou [Japon] ; Hidenori Okazaki ; Yasuko Tomono [Japon] ; Hironao Nakayama [Japon] ; Shinji Fukuda [Japon] ; Tomohisa Sakaue [Japon] ; Yuji Shirakata ; Koji Sayama ; Koji Hashimoto ; Michael Detmar [Suisse] ; Shigeki Higashiyama [Japon] ; Satoshi Hirakawa [Japon]Source :
- The Journal of Biological Chemistry [ 0021-9258 ] ; 2016.
Abstract
Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), a type I transmembrane glycoprotein, is known as one of the most specific lymphatic vessel markers in the skin. In this study, we found that the ectodomain of LYVE-1 undergoes proteolytic cleavage, and this process produces soluble LYVE-1. We further identified the cleavage site for ectodomain shedding and generated an uncleavable mutant of LYVE-1. In lymphatic endothelial cells, ectodomain shedding of LYVE-1 was induced by vascular endothelial growth factor (VEGF)-A, an important factor for angiogenesis and lymphangiogenesis under pathological conditions. VEGF-A-induced LYVE-1 ectodomain shedding was mediated via the extracellular signal-regulated kinase (ERK) and a disintegrin and metalloproteinase (ADAM) 17. Wild-type LYVE-1, but not uncleavable LYVE-1, promoted migration of lymphatic endothelial cells in response to VEGF-A. Immunostaining analyses in human psoriasis skin lesions and VEGF-A transgenic mouse skin suggested that the ectodomain shedding of LYVE-1 occurred in lymphatic vessels undergoing chronic inflammation. These results indicate that the ectodomain shedding of LYVE-1 might be involved in promoting pathological lymphangiogenesis.
Url:
DOI: 10.1074/jbc.M115.683201
PubMed: 26966180
PubMed Central: 4865900
Affiliations:
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sort="Nishida Fukuda, Hisayo" uniqKey="Nishida Fukuda H" first="Hisayo" last="Nishida-Fukuda">Hisayo Nishida-Fukuda</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation></author><author><name sortKey="Araki, Ryoichi" sort="Araki, Ryoichi" uniqKey="Araki R" first="Ryoichi" last="Araki">Ryoichi Araki</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Shudou, Masachika" sort="Shudou, Masachika" uniqKey="Shudou M" first="Masachika" last="Shudou">Masachika Shudou</name><affiliation wicri:level="1"><nlm:aff id="aff3">Bioscience, Advanced Research Support Center (ADRES), Ehime University Graduate School of Medicine, Toon 791-0295, Japan,</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Bioscience, Advanced Research Support Center (ADRES), Ehime University Graduate School of Medicine, Toon 791-0295</wicri:regionArea><wicri:noRegion>Toon 791-0295</wicri:noRegion></affiliation></author><author><name sortKey="Okazaki, Hidenori" sort="Okazaki, Hidenori" uniqKey="Okazaki H" first="Hidenori" last="Okazaki">Hidenori Okazaki</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Tomono, Yasuko" sort="Tomono, Yasuko" uniqKey="Tomono Y" first="Yasuko" last="Tomono">Yasuko Tomono</name><affiliation wicri:level="1"><nlm:aff id="aff4">Division of Molecular and Cell Biology, Shigei Medical Research Institute, Okayama 701-0202, Japan,</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Molecular and Cell Biology, Shigei Medical Research Institute, Okayama 701-0202</wicri:regionArea><wicri:noRegion>Okayama 701-0202</wicri:noRegion></affiliation></author><author><name sortKey="Nakayama, Hironao" sort="Nakayama, Hironao" uniqKey="Nakayama H" first="Hironao" last="Nakayama">Hironao Nakayama</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan,</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295, Japan,</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295</wicri:regionArea><wicri:noRegion>Toon 791-0295</wicri:noRegion></affiliation></author><author><name sortKey="Fukuda, Shinji" sort="Fukuda, Shinji" uniqKey="Fukuda S" first="Shinji" last="Fukuda">Shinji Fukuda</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295, Japan,</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295</wicri:regionArea><wicri:noRegion>Toon 791-0295</wicri:noRegion></affiliation></author><author><name sortKey="Sakaue, Tomohisa" sort="Sakaue, Tomohisa" uniqKey="Sakaue T" first="Tomohisa" last="Sakaue">Tomohisa Sakaue</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295, Japan,</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295</wicri:regionArea><wicri:noRegion>Toon 791-0295</wicri:noRegion></affiliation></author><author><name sortKey="Shirakata, Yuji" sort="Shirakata, Yuji" uniqKey="Shirakata Y" first="Yuji" last="Shirakata">Yuji Shirakata</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Sayama, Koji" sort="Sayama, Koji" uniqKey="Sayama K" first="Koji" last="Sayama">Koji Sayama</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Hashimoto, Koji" sort="Hashimoto, Koji" uniqKey="Hashimoto K" first="Koji" last="Hashimoto">Koji Hashimoto</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name><affiliation wicri:level="1"><nlm:aff wicri:cut=", and" id="aff7">Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich CH-8093, Switzerland</nlm:aff><country xml:lang="fr">Suisse</country><wicri:regionArea>Institute of Pharmaceutical Sciences, Swiss Federal Institute of Technology, ETH Zurich, Zurich CH-8093</wicri:regionArea><wicri:noRegion>Zurich CH-8093</wicri:noRegion></affiliation></author><author><name sortKey="Higashiyama, Shigeki" sort="Higashiyama, Shigeki" uniqKey="Higashiyama S" first="Shigeki" last="Higashiyama">Shigeki Higashiyama</name><affiliation><nlm:aff wicri:cut=", and" id="aff2">Biochemistry and Molecular Genetics</nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan,</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295, Japan,</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Division of Cell Growth and Tumor Regulation, Proteo-Science Center (PROS), Ehime University, Toon 791-0295</wicri:regionArea><wicri:noRegion>Toon 791-0295</wicri:noRegion></affiliation></author><author><name sortKey="Hirakawa, Satoshi" sort="Hirakawa, Satoshi" uniqKey="Hirakawa S" first="Satoshi" last="Hirakawa">Satoshi Hirakawa</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation wicri:level="1"><nlm:aff id="aff5">Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083, Japan,</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Strategic Young Researcher Overseas Visiting Program for Accelerating Brain Circulation, Japan Society for the Promotion of Science (JSPS), 5-3-1 Kojimachi, Chiyoda-ku, Tokyo 102-0083</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff8">Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu 431-3192, Japan</nlm:aff><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Dermatology, Hamamatsu University School of Medicine, Hamamatsu 431-3192</wicri:regionArea><wicri:noRegion>Hamamatsu 431-3192</wicri:noRegion></affiliation></author></analytic><series><title level="j">The Journal of Biological Chemistry</title><idno type="ISSN">0021-9258</idno><idno type="eISSN">1083-351X</idno><imprint><date when="2016">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1), a type I transmembrane glycoprotein, is known as one of the most specific lymphatic vessel markers in the skin. In this study, we found that the ectodomain of LYVE-1 undergoes proteolytic cleavage, and this process produces soluble LYVE-1. We further identified the cleavage site for ectodomain shedding and generated an uncleavable mutant of LYVE-1. In lymphatic endothelial cells, ectodomain shedding of LYVE-1 was induced by vascular endothelial growth factor (VEGF)-A, an important factor for angiogenesis and lymphangiogenesis under pathological conditions. VEGF-A-induced LYVE-1 ectodomain shedding was mediated via the extracellular signal-regulated kinase (ERK) and a disintegrin and metalloproteinase (ADAM) 17. Wild-type LYVE-1, but not uncleavable LYVE-1, promoted migration of lymphatic endothelial cells in response to VEGF-A. Immunostaining analyses in human psoriasis skin lesions and VEGF-A transgenic mouse skin suggested that the ectodomain shedding of LYVE-1 occurred in lymphatic vessels undergoing chronic inflammation. These results indicate that the ectodomain shedding of LYVE-1 might be involved in promoting pathological lymphangiogenesis.</p></div></front></TEI><affiliations><list><country><li>Japon</li><li>Suisse</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Araki, Ryoichi" sort="Araki, Ryoichi" uniqKey="Araki R" first="Ryoichi" last="Araki">Ryoichi Araki</name><name sortKey="Hashimoto, Koji" sort="Hashimoto, Koji" uniqKey="Hashimoto K" first="Koji" last="Hashimoto">Koji Hashimoto</name><name sortKey="Nishida Fukuda, Hisayo" sort="Nishida Fukuda, Hisayo" uniqKey="Nishida Fukuda H" first="Hisayo" last="Nishida-Fukuda">Hisayo Nishida-Fukuda</name><name sortKey="Okazaki, Hidenori" sort="Okazaki, Hidenori" uniqKey="Okazaki H" first="Hidenori" last="Okazaki">Hidenori Okazaki</name><name sortKey="Sayama, Koji" sort="Sayama, Koji" uniqKey="Sayama K" first="Koji" last="Sayama">Koji Sayama</name><name sortKey="Shirakata, Yuji" sort="Shirakata, Yuji" uniqKey="Shirakata Y" first="Yuji" last="Shirakata">Yuji Shirakata</name></noCountry><country name="Japon"><noRegion><name sortKey="Shudou, Masachika" sort="Shudou, Masachika" uniqKey="Shudou M" first="Masachika" last="Shudou">Masachika Shudou</name></noRegion><name sortKey="Fukuda, Shinji" sort="Fukuda, Shinji" uniqKey="Fukuda S" first="Shinji" last="Fukuda">Shinji Fukuda</name><name sortKey="Higashiyama, Shigeki" sort="Higashiyama, Shigeki" uniqKey="Higashiyama S" first="Shigeki" last="Higashiyama">Shigeki Higashiyama</name><name sortKey="Higashiyama, Shigeki" sort="Higashiyama, Shigeki" uniqKey="Higashiyama S" first="Shigeki" last="Higashiyama">Shigeki Higashiyama</name><name sortKey="Hirakawa, Satoshi" sort="Hirakawa, Satoshi" uniqKey="Hirakawa S" first="Satoshi" last="Hirakawa">Satoshi Hirakawa</name><name sortKey="Hirakawa, Satoshi" sort="Hirakawa, Satoshi" uniqKey="Hirakawa S" first="Satoshi" last="Hirakawa">Satoshi Hirakawa</name><name sortKey="Nakayama, Hironao" sort="Nakayama, Hironao" uniqKey="Nakayama H" first="Hironao" last="Nakayama">Hironao Nakayama</name><name sortKey="Nakayama, Hironao" sort="Nakayama, Hironao" uniqKey="Nakayama H" first="Hironao" last="Nakayama">Hironao Nakayama</name><name sortKey="Sakaue, Tomohisa" sort="Sakaue, Tomohisa" uniqKey="Sakaue T" first="Tomohisa" last="Sakaue">Tomohisa Sakaue</name><name sortKey="Tomono, Yasuko" sort="Tomono, Yasuko" uniqKey="Tomono Y" first="Yasuko" last="Tomono">Yasuko Tomono</name></country><country name="Suisse"><noRegion><name sortKey="Detmar, Michael" sort="Detmar, Michael" uniqKey="Detmar M" first="Michael" last="Detmar">Michael Detmar</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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